Coding
Part:BBa_K5048048:Experience
Designed by: Ziyan Peng Group: iGEM24_WHU-China (2024-09-23)
Experiments
We handed this original sequence to the biology company Tsingke and implemented condon optimization. To facilitate the detection of protein products, we added a 6xHis tag at the C-terminal of the protein forming LAP-6xHis(BBa_K5048058) and inserted this improved sequence into pET-28a backbone to create the plasmid LAP-6xHis.
Applications of BBa_K5048048
When present on the outer membrane of non-pathogenic Listeria, it acts as a beneficial protein that binds with HSP60 in the small intestine, occupying the binding site of pathogenic Listeria and avoiding the causing intestinal infection.
User Reviews
UNIQ2985839d8d648e92-partinfo-00000000-QINU UNIQ2985839d8d648e92-partinfo-00000001-QINU